› 1t3h

X-ray diffraction
2.5Å resolution

X-ray Structure of Dephospho-CoA Kinase from E. coli Norteast Structural Genomics Consortium Target ER57

Released:
DOI: 10.2210/pdb1t3h/pdb
PDB entry 1t3h coloured by chain, front view.
PDB entry 1t3h coloured by chain, side view.
PDB entry 1t3h coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Kuzin AP, Chen Y, Forouhar F, Edstrom W, Benach J, Vorobiev S, Acton T, Shastry R, Ma L-C, Xia R, Montelione G, Tong L, Hunt J, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
ATP + 3'-dephospho-CoA = ADP + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141363 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dephospho-CoA kinase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 214 amino acids
Theoretical weight: 23.86 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6I9 (Residues: 1-206; Coverage: 100%)
Gene names: JW0100, b0103, coaE, yacE
Sequence domains: Dephospho-CoA kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 55.63Å b: 80.905Å c: 75.454Å
α: 90° β: 93.95° γ: 90°
R-values:
R R work R free
0.238 0.238 0.284
Expression system: Escherichia coli

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