› 1t4c

X-ray diffraction
2.61Å resolution

Formyl-CoA Transferase in complex with Oxalyl-CoA

Released:
DOI: 10.2210/pdb1t4c/pdb
PDB entry 1t4c coloured by chain, front view.
PDB entry 1t4c coloured by chain, side view.
PDB entry 1t4c coloured by chain, top view.
Source organism: Oxalobacter formigenes
Primary publication:
Kinetic and mechanistic characterization of the formyl-CoA transferase from Oxalobacter formigenes.
Jonsson S, Ricagno S, Lindqvist Y, Richards NG
J Biol Chem 279 36003-12 (2004)
PMID: 15213226

Function and Biology Details

Reaction catalysed: 
Formyl-CoA + oxalate = formate + oxalyl-CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126525 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formyl-CoA:oxalate CoA-transferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 427 amino acids
Theoretical weight: 47.23 KDa
Source organism: Oxalobacter formigenes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O06644 (Residues: 2-428; Coverage: 100%)
Gene name: frc
Sequence domains: CoA-transferase family III
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand COA 2 x COA
Ligand OXD 1 x OXD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P43212
Unit cell:
a: 100.163Å b: 100.163Å c: 196.872Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.2 0.273
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

The enzymes of oxalate metabolism: unexpected structures and mechanisms.
Svedruzić et al. (2005)
3 more

1 mention without citation

Analysis of Essential Isoprene Metabolic Pathway Proteins in Variovorax sp. Strain WS11.
Rix et al. (2023)