› 1t5e

X-ray diffraction
3Å resolution

The structure of MexA

Released:
DOI: 10.2210/pdb1t5e/pdb
PDB entry 1t5e coloured by chain, front view.
PDB entry 1t5e coloured by chain, side view.
PDB entry 1t5e coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structure of the periplasmic component of a bacterial drug efflux pump.
Higgins MK, Bokma E, Koronakis E, Hughes C, Koronakis V
Proc Natl Acad Sci U S A 101 9994-9 (2004)
PMID: 15226509

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tridecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156553 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug resistance protein MexA Chains: A, B, C, D, E, F, G, H, I, J, K, L, M
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M
Length: 360 amino acids
Theoretical weight: 38.63 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P52477 (Residues: 24-383; Coverage: 100%)
Gene names: PA0425, mexA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 4 x GOL
Ligand 3GR 9 x 3GR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 130.551Å b: 183.585Å c: 213.314Å
α: 90° β: 107.38° γ: 90°
R-values:
R R work R free
0.274 0.273 0.285
Expression system: Escherichia coli

Quick links

Citations

46 review citations

Structure, function, and evolution of bacterial ATP-binding cassette systems.
Davidson et al. (2008)
45 more

9 mentions without citation

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)
8 more