› 1t6j

X-ray diffraction
2.1Å resolution

Crystal Structure of Phenylalanine Ammonia Lyase from Rhodosporidium toruloides

Released:
DOI: 10.2210/pdb1t6j/pdb
PDB entry 1t6j coloured by chain, front view.
PDB entry 1t6j coloured by chain, side view.
PDB entry 1t6j coloured by chain, top view.
Source organism: Rhodotorula toruloides
Primary publication:
Crystal structure of phenylalanine ammonia lyase: multiple helix dipoles implicated in catalysis.
Calabrese JC, Jordan DB, Boodhoo A, Sariaslani S, Vannelli T
Biochemistry 43 11403-16 (2004)
PMID: 15350127

Function and Biology Details

Reaction catalysed: 
L-phenylalanine = trans-cinnamate + ammonia
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145872 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenylalanine/tyrosine ammonia-lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 714 amino acids
Theoretical weight: 77.66 KDa
Source organism: Rhodotorula toruloides
Expression system: Escherichia coli
UniProt:
  • Canonical: P11544 (Residues: 1-716; Coverage: 100%)
Gene name: PAL
Sequence domains: Aromatic amino acid lyase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CIN 1 x CIN
2 modified residues:
Ligand MSE 30 x MSE
Ligand 175 2 x 175

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P3221
Unit cell:
a: 107.86Å b: 107.86Å c: 204.38Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.251 0.243 0.298
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Protein posttranslational modifications: the chemistry of proteome diversifications.
Walsh et al. (2005)
20 more

5 mentions without citation

Structural and biochemical characterization of the therapeutic Anabaena variabilis phenylalanine ammonia lyase.
Wang et al. (2008)
4 more