› 1t6l

X-ray diffraction
1.85Å resolution

Crystal Structure of the Human Cytomegalovirus DNA Polymerase Subunit, UL44

Released:
DOI: 10.2210/pdb1t6l/pdb
PDB entry 1t6l coloured by chain, front view.
PDB entry 1t6l coloured by chain, side view.
PDB entry 1t6l coloured by chain, top view.
Source organism: Human betaherpesvirus 5
Primary publication:
The cytomegalovirus DNA polymerase subunit UL44 forms a C clamp-shaped dimer.
Appleton BA, Loregian A, Filman DJ, Coen DM, Hogle JM
Mol Cell 15 233-44 (2004)
PMID: 15260974

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147814 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase processivity factor Chain: A
Molecule details ›
Chain: A
Length: 290 amino acids
Theoretical weight: 32.58 KDa
Source organism: Human betaherpesvirus 5
Expression system: Escherichia coli
UniProt:
  • Canonical: P16790 (Residues: 1-290; Coverage: 67%)
Gene name: UL44
Sequence domains: Herpesvirus polymerase accessory protein
Structure domains: Proliferating Cell Nuclear Antigen

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P6122
Unit cell:
a: 53.93Å b: 53.93Å c: 340.067Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.238 0.235 0.268
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Herpes simplex viruses: mechanisms of DNA replication.
Weller et al. (2012)
9 more

9 mentions without citation

The flexible loop of the human cytomegalovirus DNA polymerase processivity factor ppUL44 is required for efficient DNA binding and replication in cells.
Alvisi et al. (2009)
8 more