› 1t6p

X-ray diffraction
2.7Å resolution

Crystal Structure of Phenylalanine Ammonia Lyase from Rhodosporidium toruloides

Released:
DOI: 10.2210/pdb1t6p/pdb
PDB entry 1t6p coloured by chain, front view.
PDB entry 1t6p coloured by chain, side view.
PDB entry 1t6p coloured by chain, top view.
Source organism: Rhodotorula toruloides
Primary publication:
Crystal structure of phenylalanine ammonia lyase: multiple helix dipoles implicated in catalysis.
Calabrese JC, Jordan DB, Boodhoo A, Sariaslani S, Vannelli T
Biochemistry 43 11403-16 (2004)
PMID: 15350127

Function and Biology Details

Reaction catalysed: 
L-phenylalanine = trans-cinnamate + ammonia
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145872 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenylalanine/tyrosine ammonia-lyase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 714 amino acids
Theoretical weight: 77.66 KDa
Source organism: Rhodotorula toruloides
Expression system: Escherichia coli
UniProt:
  • Canonical: P11544 (Residues: 1-716; Coverage: 100%)
Gene name: PAL
Sequence domains: Aromatic amino acid lyase
Structure domains:

Ligands and Environments

No bound ligands
2 modified residues:
Ligand MSE 120 x MSE
Ligand 175 8 x 175

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P21
Unit cell:
a: 105.192Å b: 180.973Å c: 149.215Å
α: 90° β: 96.949° γ: 90°
R-values:
R R work R free
0.194 0.194 0.253
Expression system: Escherichia coli

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Citations

21 review citations

Protein posttranslational modifications: the chemistry of proteome diversifications.
Walsh et al. (2005)
20 more

5 mentions without citation

Discovery of two cyanobacterial phenylalanine ammonia lyases: kinetic and structural characterization.
Moffitt et al. (2007)
4 more