› 1t8g

X-ray diffraction
1.8Å resolution

Crystal structure of phage T4 lysozyme mutant L32A/L33A/T34A/C54T/C97A/E108V

Released:
DOI: 10.2210/pdb1t8g/pdb
PDB entry 1t8g coloured by chain, front view.
PDB entry 1t8g coloured by chain, side view.
PDB entry 1t8g coloured by chain, top view.
Source organism: Escherichia virus T4
Primary publication:
Alanine-scanning mutagenesis of the beta-sheet region of phage T4 lysozyme suggests that tertiary context has a dominant effect on beta-sheet formation.
He MM, Wood ZA, Baase WA, Xiao H, Matthews BW
Protein Sci 13 2716-24 (2004)
PMID: 15340171

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-133020 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 18.51 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P00720 (Residues: 1-164; Coverage: 100%)
Gene name: E
Sequence domains: Phage lysozyme
Structure domains: Lysozyme

Ligands and Environments

3 bound ligands:
Ligand PO4 1 x PO4
Ligand CL 2 x CL
Ligand BME 1 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: I422
Unit cell:
a: 96.037Å b: 96.037Å c: 77.265Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.184 0.215
Expression system: Escherichia coli

Quick links

Citations

12 citation in other articles

An approach to crystallizing proteins by synthetic symmetrization.
Banatao et al. (2006)
11 more

1 mention without citation

Alanine-scanning mutagenesis of the beta-sheet region of phage T4 lysozyme suggests that tertiary context has a dominant effect on beta-sheet formation.
He et al. (2004)