› 1t8w

X-ray diffraction
2.8Å resolution

Crystal Structure of E. coli AMP Nucleosidase

Released:
DOI: 10.2210/pdb1t8w/pdb
PDB entry 1t8w coloured by chain, front view.
PDB entry 1t8w coloured by chain, side view.
PDB entry 1t8w coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of Escherichia coli AMP nucleosidase reveals similarity to nucleoside phosphorylases.
Zhang Y, Cottet SE, Ealick SE
Structure 12 1383-94 (2004)
PMID: 15296732

Function and Biology Details

Reaction catalysed: 
AMP + H(2)O = D-ribose 5-phosphate + adenine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142396 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AMP nucleosidase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 484 amino acids
Theoretical weight: 54.24 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AE12 (Residues: 1-484; Coverage: 100%)
Gene names: JW1963, amn, b1982
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 8-BM
Spacegroup: I4
Unit cell:
a: 271.8Å b: 271.8Å c: 113.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.223 0.245
Expression system: Escherichia coli

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Citations

4 review citations

Transition States, analogues, and drug development.
Schramm VL. (2013)
3 more