› 1t90

X-ray diffraction
2.5Å resolution

Crystal structure of methylmalonate semialdehyde dehydrogenase from Bacillus subtilis

Released:
DOI: 10.2210/pdb1t90/pdb
PDB entry 1t90 coloured by chain, front view.
PDB entry 1t90 coloured by chain, side view.
PDB entry 1t90 coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Dubourg H, Didierjean C, Stines-Chaumeil C, Talfournier F, Branlant G, Aubry A, Corbier C

Function and Biology Details

Reaction catalysed: 
2-methyl-3-oxopropanoate + CoA + H(2)O + NAD(+) = propanoyl-CoA + HCO(3)(-) + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154648 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malonate-semialdehyde dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 486 amino acids
Theoretical weight: 53.38 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P42412 (Residues: 2-487; Coverage: 100%)
Gene names: BSU39760, E83A, iolA, mmsA, yxdA
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P212121
Unit cell:
a: 195.2Å b: 192.5Å c: 83.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.25
Expression system: Escherichia coli

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