› 1t9z

X-ray diffraction
2.3Å resolution

Three-dimensional structure of a RNA-polymerase II binding protein.

Released:
DOI: 10.2210/pdb1t9z/pdb
PDB entry 1t9z coloured by chain, front view.
PDB entry 1t9z coloured by chain, side view.
PDB entry 1t9z coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and mechanism of RNA polymerase II CTD phosphatases.
Kamenski T, Heilmeier S, Meinhart A, Cramer P
Mol Cell 15 399-407 (2004)
PMID: 15304220

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190456 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1 Chain: A
Molecule details ›
Chain: A
Length: 197 amino acids
Theoretical weight: 22.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9GZU7 (Residues: 77-261; Coverage: 71%)
Gene names: CTDSP1, NIF3, NLIIF, SCP1
Sequence domains: NLI interacting factor-like phosphatase
Structure domains: HAD superfamily/HAD-like

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand CIT 1 x CIT
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21212
Unit cell:
a: 117.55Å b: 47.19Å c: 39.98Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.187 0.234
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Serine/threonine phosphatases: mechanism through structure.
Shi Y. (2009)
21 more

1 mention without citation

Targeting the C-Terminal Domain Small Phosphatase 1.
Rallabandi et al. (2020)