› 1ta4

X-ray diffraction
2.28Å resolution

Crystal Structure Of Aspartate-Semialdehyde Dehydrogenase From Haemophilus Influenzae with a Bound Arsenate

Released:
DOI: 10.2210/pdb1ta4/pdb
PDB entry 1ta4 coloured by chain, front view.
PDB entry 1ta4 coloured by chain, side view.
PDB entry 1ta4 coloured by chain, top view.
Source organism: Haemophilus influenzae
Primary publication:
Structural basis for discrimination between oxyanion substrates or inhibitors in aspartate-beta-semialdehyde dehydrogenase.
Faehnle CR, Blanco J, Viola RE
Acta Crystallogr D Biol Crystallogr 60 2320-4 (2004)
PMID: 15583380

Function and Biology Details

Reaction catalysed: 
L-aspartate 4-semialdehyde + phosphate + NADP(+) = L-4-aspartyl phosphate + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155210 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartate-semialdehyde dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 371 amino acids
Theoretical weight: 40.58 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P44801 (Residues: 1-371; Coverage: 100%)
Gene names: HI_0646, asd
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ART 2 x ART
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21212
Unit cell:
a: 113.769Å b: 54.843Å c: 57.487Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.225 0.286
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

The molecular basis of phosphate discrimination in arsenate-rich environments.
Elias et al. (2012)
4 more

3 mentions without citation

Arsenate replacing phosphate: alternative life chemistries and ion promiscuity.
Tawfik et al. (2011)
2 more