› 1tad

X-ray diffraction
1.7Å resolution

GTPASE MECHANISM OF GPROTEINS FROM THE 1.7-ANGSTROM CRYSTAL STRUCTURE OF TRANSDUCIN ALPHA-GDP-ALF4-

Released:
DOI: 10.2210/pdb1tad/pdb
PDB entry 1tad coloured by chain, front view.
PDB entry 1tad coloured by chain, side view.
PDB entry 1tad coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
GTPase mechanism of Gproteins from the 1.7-A crystal structure of transducin alpha-GDP-AIF-4.
Sondek J, Lambright DG, Noel JP, Hamm HE, Sigler PB
Nature 372 276-9 (1994)
PMID: 7969474

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138153 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanine nucleotide-binding protein G(t) subunit alpha-1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 324 amino acids
Theoretical weight: 37.14 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P04695 (Residues: 27-350; Coverage: 93%)
Gene name: GNAT1
Sequence domains: G-protein alpha subunit
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand CA 3 x CA
Ligand CAC 6 x CAC
Ligand ALF 3 x ALF
Ligand GDP 3 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 74.9Å b: 108.2Å c: 79Å
α: 90° β: 111.7° γ: 90°
R-values:
R R work R free
0.209 0.209 0.266
Expression system: Not provided

Quick links

Citations

82 review citations

GTPase-activating proteins for heterotrimeric G proteins: regulators of G protein signaling (RGS) and RGS-like proteins.
Ross et al. (2000)
81 more

20 mentions without citation

G-protein signaling: back to the future.
McCudden et al. (2005)
19 more