› 1tbj

X-ray diffraction
2.8Å resolution

H141A mutant of rat liver arginase I

Released:
DOI: 10.2210/pdb1tbj/pdb
PDB entry 1tbj coloured by chain, front view.
PDB entry 1tbj coloured by chain, side view.
PDB entry 1tbj coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Probing the role of the hyper-reactive histidine residue of arginase.
Colleluori DM, Reczkowski RS, Emig FA, Cama E, Cox JD, Scolnick LR, Compher K, Jude K, Han S, Viola RE, Christianson DW, Ash DE
Arch Biochem Biophys 444 15-26 (2005)
PMID: 16266687

Function and Biology Details

Reaction catalysed: 
L-arginine + H(2)O = L-ornithine + urea
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139779 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arginase-1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 314 amino acids
Theoretical weight: 33.97 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P07824 (Residues: 6-319; Coverage: 97%)
Gene name: Arg1
Sequence domains: Arginase family
Structure domains: Ureohydrolase domain

Ligands and Environments

2 bound ligands:
Ligand MN 6 x MN
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P32
Unit cell:
a: 87.815Å b: 87.815Å c: 110.916Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.263 0.263 0.299
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Arginase Inhibitors: A Rational Approach Over One Century.
Pudlo et al. (2017)
8 other articles