› 1tc1

X-ray diffraction
1.41Å resolution

A 1.4 ANGSTROM CRYSTAL STRUCTURE FOR THE HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE OF TRYPANOSOMA CRUZI

Released:
DOI: 10.2210/pdb1tc1/pdb
PDB entry 1tc1 coloured by chain, front view.
PDB entry 1tc1 coloured by chain, side view.
PDB entry 1tc1 coloured by chain, top view.
Source organism: Trypanosoma cruzi
Primary publication:
A 1.4 A crystal structure for the hypoxanthine phosphoribosyltransferase of Trypanosoma cruzi.
Focia PJ, Craig SP, Nieves-Alicea R, Fletterick RJ, Eakin AE
Biochemistry 37 15066-75 (1998)
PMID: 9790669

Function and Biology Details

Reaction catalysed: 
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175481 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxanthine phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 220 amino acids
Theoretical weight: 25.46 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4DRC4 (Residues: 22-241; Coverage: 91%)
Gene name: Tc00.1047053509693.70
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand FMB 2 x FMB
Ligand MES 2 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 39.5Å b: 94.09Å c: 52.12Å
α: 90° β: 94.75° γ: 90°
R-values:
R R work R free
0.193 0.198 0.228
Expression system: Escherichia coli

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Citations

24 citation in other articles

Dissecting subunit interfaces in homodimeric proteins.
Bahadur et al. (2003)
23 more

7 mentions without citation

The Potential of Secondary Metabolites from Plants as Drugs or Leads against Protozoan Neglected Diseases-Part III: In-Silico Molecular Docking Investigations.
Ogungbe et al. (2016)
6 more