› 1tc2

X-ray diffraction
1.81Å resolution

TERNARY SUBSTRATE COMPLEX OF THE HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE FROM TRYPANOSOMA CRUZI

Released:
DOI: 10.2210/pdb1tc2/pdb
PDB entry 1tc2 coloured by chain, front view.
PDB entry 1tc2 coloured by chain, side view.
PDB entry 1tc2 coloured by chain, top view.
Source organism: Trypanosoma cruzi
Primary publication:
Approaching the transition state in the crystal structure of a phosphoribosyltransferase.
Focia PJ, Craig SP, Eakin AE
Biochemistry 37 17120-7 (1998)
PMID: 9860824

Function and Biology Details

Reaction catalysed: 
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175481 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxanthine phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 221 amino acids
Theoretical weight: 25.6 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4DRC4 (Residues: 21-241; Coverage: 92%)
Gene name: Tc00.1047053509693.70
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

4 bound ligands:
Ligand MG 3 x MG
Ligand 7HP 2 x 7HP
Ligand PRP 2 x PRP
Ligand MN 1 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 39.42Å b: 101.76Å c: 51.92Å
α: 90° β: 94.23° γ: 90°
R-values:
R R work R free
0.191 0.183 0.232
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The importance of disordered loops in ABO glycosyltransferases.
Yazer et al. (2005)
28 other articles

7 mentions without citation

The Potential of Secondary Metabolites from Plants as Drugs or Leads against Protozoan Neglected Diseases-Part III: In-Silico Molecular Docking Investigations.
Ogungbe et al. (2016)
6 more