› 1tc3

X-ray diffraction
2.45Å resolution

TRANSPOSASE TC3A1-65 FROM CAENORHABDITIS ELEGANS

Released:
DOI: 10.2210/pdb1tc3/pdb
PDB entry 1tc3 coloured by chain, front view.
PDB entry 1tc3 coloured by chain, side view.
PDB entry 1tc3 coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
Crystal structure of the specific DNA-binding domain of Tc3 transposase of C.elegans in complex with transposon DNA.
van Pouderoyen G, Ketting RF, Perrakis A, Plasterk RH, Sixma TK
EMBO J 16 6044-54 (1997)
PMID: 9312061

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114639 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transposable element Tc3 transposase Chain: C
Molecule details ›
Chain: C
Length: 51 amino acids
Theoretical weight: 5.81 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: P34257 (Residues: 2-52; Coverage: 16%)
Gene names: B0303.5, tc3a
Sequence domains: Tc3 transposase
Structure domains: Homeodomain-like
DNA (5'-D(*AP*GP*GP*GP*GP*GP*GP*GP*TP*CP*CP*TP*AP*TP*AP*GP*A P*AP*CP*TP*T)-3') Chain: A
Molecule details ›
Chain: A
Length: 21 nucleotides
Theoretical weight: 6.54 KDa
Source organism: Caenorhabditis elegans
Expression system: Not provided
DNA (5'-D(*AP*GP*TP*TP*CP*TP*AP*TP*AP*GP*GP*AP*CP*CP*CP*CP*C P*CP*CP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 20 nucleotides
Theoretical weight: 6.03 KDa
Source organism: Caenorhabditis elegans
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: C2221
Unit cell:
a: 37.18Å b: 202.79Å c: 62.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 not available 0.318
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

Resident aliens: the Tc1/mariner superfamily of transposable elements.
Plasterk et al. (1999)
6 more

17 mentions without citation

An overview of the structures of protein-DNA complexes.
Luscombe et al. (2000)
16 more