› 1tcs

X-ray diffraction
1.7Å resolution

CRYSTAL STRUCTURE OF TRICHOSANTHIN-NADPH COMPLEX AT 1.7 ANGSTROMS RESOLUTION REVEALS ACTIVE-SITE ARCHITECTURE

Released:
DOI: 10.2210/pdb1tcs/pdb
PDB entry 1tcs coloured by chain, front view.
PDB entry 1tcs coloured by chain, side view.
PDB entry 1tcs coloured by chain, top view.
Source organism: Trichosanthes kirilowii
Primary publication:
Crystal structure of trichosanthin-NADPH complex at 1.7 A resolution reveals active-site architecture.
Xiong JP, Xia ZX, Wang Y
Nat Struct Biol 1 695-700 (1994)
PMID: 7634073

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140879 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosome-inactivating protein alpha-trichosanthin Chain: A
Molecule details ›
Chain: A
Length: 247 amino acids
Theoretical weight: 27.17 KDa
Source organism: Trichosanthes kirilowii
Expression system: Not provided
UniProt:
  • Canonical: P09989 (Residues: 24-270; Coverage: 93%)
Sequence domains: Ribosome inactivating protein
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NDP 1 x NDP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 38.39Å b: 76.81Å c: 79.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 not available not available
Expression system: Not provided

Quick links

Citations

2 review citations

Biological activities of ribosome-inactivating proteins and their possible applications as antimicrobial, anticancer, and anti-pest agents and in neuroscience research.
Akkouh et al. (2015)
1 more

8 mentions without citation

Strategies to Improve the Clinical Utility of Saporin-Based Targeted Toxins.
Giansanti et al. (2018)
7 more