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X-ray diffraction
2.6Å resolution

Mycobacterium smegmatis Stf0 Sulfotransferase with Trehalose

Released:
DOI: 10.2210/pdb1tex/pdb
PDB entry 1tex coloured by chain, front view.
PDB entry 1tex coloured by chain, side view.
PDB entry 1tex coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis
Primary publication:
Identification, function and structure of the mycobacterial sulfotransferase that initiates sulfolipid-1 biosynthesis.
Mougous JD, Petzold CJ, Senaratne RH, Lee DH, Akey DL, Lin FL, Munchel SE, Pratt MR, Riley LW, Leary JA, Berger JM, Bertozzi CR
Nat Struct Mol Biol 11 721-9 (2004)
PMID: 15258569

Function and Biology Details

Reaction catalysed: 
3'-phosphoadenylyl sulfate + alpha,alpha-trehalose = adenosine 3',5'-bisphosphate + 2-O-sulfo-alpha,alpha-trehalose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160849 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Trehalose 2-sulfotransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 287 amino acids
Theoretical weight: 32.5 KDa
Source organism: Mycolicibacterium smegmatis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P84151 (Residues: 1-267; Coverage: 100%)
Gene name: NCTC7017_01453
Sequence domains: Stf0 sulphotransferase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2, ALS BEAMLINE 8.3.1
Spacegroup: P3221
Unit cell:
a: 101.929Å b: 101.929Å c: 228.212Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.218 0.274
Expression system: Escherichia coli BL21

Quick links

Citations

19 review citations

Virulence factors of the Mycobacterium tuberculosis complex.
Forrellad et al. (2013)
18 more

2 mentions without citation

SIFTS: updated Structure Integration with Function, Taxonomy and Sequences resource allows 40-fold increase in coverage of structure-based annotations for proteins.
Dana et al. (2019)
1 more