› 1tf7

X-ray diffraction
2.8Å resolution

Crystal Structure of Circadian Clock Protein KaiC

Released:
DOI: 10.2210/pdb1tf7/pdb
PDB entry 1tf7 coloured by chain, front view.
PDB entry 1tf7 coloured by chain, side view.
PDB entry 1tf7 coloured by chain, top view.
Source organism: Synechococcus sp.
Primary publication:
Visualizing a circadian clock protein: crystal structure of KaiC and functional insights.
Pattanayek R, Wang J, Mori T, Xu Y, Johnson CH, Egli M
Mol Cell 15 375-88 (2004)
PMID: 15304218

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181278 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Circadian clock oscillator protein KaiC Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 525 amino acids
Theoretical weight: 58.9 KDa
Source organism: Synechococcus sp.
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q79PF4 (Residues: 1-519; Coverage: 100%)
Gene names: Synpcc7942_1216, kaiC, see0011
Sequence domains: KaiC
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand ATP 12 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B, APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 132.873Å b: 135.576Å c: 204.951Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.24 0.28
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

27 review citations

Circadian Rhythms in Cyanobacteria.
Cohen et al. (2015)
26 more

7 mentions without citation

Architecture and mechanism of the central gear in an ancient molecular timer.
Egli M. (2017)
6 more