› 1tfb

Solution NMR

NMR STUDIES OF HUMAN GENERAL TRANSCRIPTION FACTOR TFIIB: DYNAMICS AND INTERACTION WITH VP16 ACTIVATION DOMAIN, 20 STRUCTURES

Released:
DOI: 10.2210/pdb1tfb/pdb
PDB entry 1tfb coloured by chain, ensemble of 20 models, front view.
PDB entry 1tfb coloured by chain, ensemble of 20 models, side view.
PDB entry 1tfb coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Human general transcription factor TFIIB: conformational variability and interaction with VP16 activation domain.
Hayashi F, Ishima R, Liu D, Tong KI, Kim S, Reinberg D, Bagby S, Ikura M
Biochemistry 37 7941-51 (1998)
PMID: 9609687

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169410 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription initiation factor IIB Chain: A
Molecule details ›
Chain: A
Length: 208 amino acids
Theoretical weight: 23.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q00403 (Residues: 110-316; Coverage: 66%)
Gene names: GTF2B, TF2B, TFIIB
Sequence domains: Transcription factor TFIIB repeat
Structure domains: Cyclin-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Structural basis of transcription initiation by RNA polymerase II.
Sainsbury et al. (2015)
5 more

3 mentions without citation

An automated stochastic approach to the identification of the protein specificity determinants and functional subfamilies.
Mazin et al. (2010)
2 more