› 1tgy

X-ray diffraction
2.2Å resolution

Structure of E. coli Uridine Phosphorylase complexed with uracil and ribose 1-phosphate

Released:
DOI: 10.2210/pdb1tgy/pdb
PDB entry 1tgy coloured by chain, front view.
PDB entry 1tgy coloured by chain, side view.
PDB entry 1tgy coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Bu W, Settembre EC, Sanders JM, Begley TP, Ealick SE

Function and Biology Details

Reaction catalysed: 
Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146313 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridine phosphorylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 256 amino acids
Theoretical weight: 27.47 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P12758 (Residues: 1-253; Coverage: 100%)
Gene names: JW3808, b3831, udp
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

3 bound ligands:
Ligand R1P 2 x R1P
Ligand K 1 x K
Ligand URA 2 x URA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: R3
Unit cell:
a: 149.972Å b: 149.972Å c: 49.566Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.16 0.16 0.211
Expression system: Escherichia coli K-12

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