› 1thg

X-ray diffraction
1.8Å resolution

1.8 ANGSTROMS REFINED STRUCTURE OF THE LIPASE FROM GEOTRICHUM CANDIDUM

Released:
DOI: 10.2210/pdb1thg/pdb
PDB entry 1thg coloured by chain, front view.
PDB entry 1thg coloured by chain, side view.
PDB entry 1thg coloured by chain, top view.
Source organism: Geotrichum candidum
Primary publication:
1.8 A refined structure of the lipase from Geotrichum candidum.
Schrag JD, Cygler M
J Mol Biol 230 575-91 (1993)
PMID: 8464065

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149623 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lipase 2 Chain: A
Molecule details ›
Chain: A
Length: 544 amino acids
Theoretical weight: 59.73 KDa
Source organism: Geotrichum candidum
Expression system: Not provided
UniProt:
  • Canonical: P22394 (Residues: 21-563; Coverage: 100%)
Gene name: LIP2
Sequence domains: Carboxylesterase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NAG 1 x NAG
Ligand NDG 1 x NDG
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 59.4Å b: 84Å c: 56Å
α: 90° β: 100.1° γ: 90°
R-values:
R R work R free
0.157 0.157 not available
Expression system: Not provided

Quick links

Citations

9 review citations

Of barn owls and bankers: a lush variety of alpha/beta hydrolases.
Heikinheimo et al. (1999)
8 more

15 mentions without citation

Structural biology of retroviral DNA integration.
Li et al. (2011)
14 more