› 1tjg

X-ray diffraction
2Å resolution

Crystal Structure of the broadly neutralizing anti-HIV-1 antibody 2F5 in complex with a gp41 7mer epitope

Released:
DOI: 10.2210/pdb1tjg/pdb
PDB entry 1tjg coloured by chain, front view.
PDB entry 1tjg coloured by chain, side view.
PDB entry 1tjg coloured by chain, top view.
Source organisms:
Primary publication:
Structure and mechanistic analysis of the anti-human immunodeficiency virus type 1 antibody 2F5 in complex with its gp41 epitope.
Ofek G, Tang M, Sambor A, Katinger H, Mascola JR, Wyatt R, Kwong PD
J Virol 78 10724-37 (2004)
PMID: 15367639

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212451 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
FAB 2F5 Light Chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.36 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
FAB 2F5 Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 237 amino acids
Theoretical weight: 25.29 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
Transmembrane protein gp41 Chain: P
Molecule details ›
Chain: P
Length: 7 amino acids
Theoretical weight: 849 Da
Source organism: Human immunodeficiency virus 1
Expression system: Not provided
UniProt:
  • Canonical: Q75760 (Residues: 653-659; Coverage: 1%)
Gene name: env

Ligands and Environments

2 bound ligands:
Ligand EDO 3 x EDO
Ligand IPA 7 x IPA
1 modified residue:
Ligand YCM 2 x YCM

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 57.86Å b: 64.02Å c: 179.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.225
Expression systems:
  • Cricetulus griseus
  • Not provided

Quick links

Citations

77 review citations

Structures and mechanisms of viral membrane fusion proteins: multiple variations on a common theme.
White et al. (2008)
76 more

17 mentions without citation

Neutralizing ebolavirus: structural insights into the envelope glycoprotein and antibodies targeted against it.
Lee et al. (2009)
16 more