› 1tkv

Solution NMR

Solution Structure of T4 AsiA Dimer

Released:
DOI: 10.2210/pdb1tkv/pdb
PDB entry 1tkv coloured by chain, ensemble of 23 models, front view.
PDB entry 1tkv coloured by chain, ensemble of 23 models, side view.
PDB entry 1tkv coloured by chain, ensemble of 23 models, top view.
Source organism: Escherichia virus T4
Primary publication:
Flipping a genetic switch by subunit exchange.
Lambert LJ, Schirf V, Demeler B, Cadene M, Werner MH
EMBO J 20 7149-59 (2001)
PMID: 11742991

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152249 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
10 kDa anti-sigma factor Chains: A, B
Molecule details ›
Chains: A, B
Length: 90 amino acids
Theoretical weight: 10.6 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P32267 (Residues: 1-90; Coverage: 100%)
Gene name: asiA
Sequence domains: Anti-Sigma Factor A
Structure domains: Anti-Sigma Factor A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: dynamical simulated annealing/restrained energy minimization
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Bacteriophage T4 genome.
Miller et al. (2003)
3 more

3 mentions without citation

Traditional biomolecular structure determination by NMR spectroscopy allows for major errors.
Nabuurs et al. (2006)
2 more