› 1tnz

X-ray diffraction
2.9Å resolution

Rat Protein Geranylgeranyltransferase Type-I Complexed with a GGPP analog and a RRCVLL Peptide Derived from Cdc42 splice isoform-2

Released:
DOI: 10.2210/pdb1tnz/pdb
PDB entry 1tnz coloured by chain, front view.
PDB entry 1tnz coloured by chain, side view.
PDB entry 1tnz coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Crystallographic analysis of CaaX prenyltransferases complexed with substrates defines rules of protein substrate selectivity.
Reid TS, Terry KL, Casey PJ, Beese LS
J Mol Biol 343 417-33 (2004)
PMID: 15451670

Function and Biology Details

Reactions catalysed: 
Farnesyl diphosphate + protein-cysteine = S-farnesyl protein + diphosphate
Geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-156826 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 377 amino acids
Theoretical weight: 44.1 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q04631 (Residues: 1-377; Coverage: 100%)
Gene name: Fnta
Sequence domains: Protein prenyltransferase alpha subunit repeat
Structure domains: Protein prenylyltransferase
Geranylgeranyl transferase type-1 subunit beta Chains: B, D, F, H, J, L
Molecule details ›
Chains: B, D, F, H, J, L
Length: 377 amino acids
Theoretical weight: 42.47 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P53610 (Residues: 1-377; Coverage: 100%)
Gene name: Pggt1b
Sequence domains: Prenyltransferase and squalene oxidase repeat
Structure domains: Glycosyltransferase
Cell division control protein 42 homolog (Cdc42) Chains: M, N, O, P, Q, R
Molecule details ›
Chains: M, N, O, P, Q, R
Length: 6 amino acids
Theoretical weight: 761 Da

Ligands and Environments

4 bound ligands:
Ligand ZN 6 x ZN
Ligand MGM 6 x MGM
Ligand CL 3 x CL
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2
Unit cell:
a: 271.262Å b: 266.922Å c: 185.75Å
α: 90° β: 131.91° γ: 90°
R-values:
R R work R free
0.199 0.199 0.218
Expression system: Spodoptera frugiperda

Quick links

Citations

36 review citations

Targeting the Raf-MEK-ERK mitogen-activated protein kinase cascade for the treatment of cancer.
Roberts et al. (2007)
35 more