› 1to0

X-ray diffraction
2.5Å resolution

X-ray structure of Northeast Structural Genomics target protein sr145 from Bacillus subtilis

Released:
DOI: 10.2210/pdb1to0/pdb
PDB entry 1to0 coloured by chain, front view.
PDB entry 1to0 coloured by chain, side view.
PDB entry 1to0 coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Kuzin AP, Edstrom W, Vorobiev SM, Shastry R, Ma L-C, Xiao R, Acton T, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + pseudouridine(1915) in 23S rRNA = S-adenosyl-L-homocysteine + N(3)-methylpseudouridine(1915) in 23S rRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175063 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA large subunit methyltransferase H Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 167 amino acids
Theoretical weight: 19.46 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q45601 (Residues: 1-159; Coverage: 100%)
Gene names: BSU40230, rlmH, yydA
Sequence domains: Predicted SPOUT methyltransferase
Structure domains: Alpha/beta knot

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 48 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 98.691Å b: 70.553Å c: 100.488Å
α: 90° β: 95.16° γ: 90°
R-values:
R R work R free
0.237 0.237 0.29
Expression system: Escherichia coli BL21(DE3)

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