› 1tox

X-ray diffraction
2.3Å resolution

DIPHTHERIA TOXIN DIMER COMPLEXED WITH NAD

Released:
DOI: 10.2210/pdb1tox/pdb
PDB entry 1tox coloured by chain, front view.
PDB entry 1tox coloured by chain, side view.
PDB entry 1tox coloured by chain, top view.
Source organism: Corynephage beta
Primary publication:
Crystal structure of diphtheria toxin bound to nicotinamide adenine dinucleotide.
Bell CE, Eisenberg D
Biochemistry 35 1137-49 (1996)
PMID: 8573568

Function and Biology Details

Reaction catalysed: 
NAD(+) + diphthamide-[translation elongation factor 2] = nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132742 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Diphtheria toxin Chains: A, B
Molecule details ›
Chains: A, B
Length: 535 amino acids
Theoretical weight: 58.41 KDa
Source organism: Corynephage beta
UniProt:
  • Canonical: P00588 (Residues: 33-567; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 105Å b: 89.5Å c: 130.1Å
α: 90° β: 94° γ: 90°
R-values:
R R work R free
0.227 0.227 0.307

Quick links

Citations

38 review citations

New insights into the molecular and cellular functions of poly(ADP-ribose) and PARPs.
Gibson et al. (2012)
37 more

28 mentions without citation

The PARP side of the nucleus: molecular actions, physiological outcomes, and clinical targets.
Krishnakumar et al. (2010)
27 more