› 1tp7

X-ray diffraction
2.4Å resolution

Crystal Structure of the RNA-dependent RNA Polymerase from Human Rhinovirus 16

Released:
DOI: 10.2210/pdb1tp7/pdb
PDB entry 1tp7 coloured by chain, front view.
PDB entry 1tp7 coloured by chain, side view.
PDB entry 1tp7 coloured by chain, top view.
Source organism: rhinovirus A16
Primary publication:
Crystal structure of complete rhinovirus RNA polymerase suggests front loading of protein primer.
Appleby TC, Luecke H, Shim JH, Wu JZ, Cheney IW, Zhong W, Vogeley L, Hong Z, Yao N
J Virol 79 277-88 (2005)
PMID: 15596823

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182635 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 460 amino acids
Theoretical weight: 52.81 KDa
Source organism: rhinovirus A16
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q82122 (Residues: 1694-2153; Coverage: 21%)
Sequence domains: Viral RNA-dependent RNA polymerase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand DMX 1 x DMX
1 modified residue:
Ligand MSE 40 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 59.428Å b: 118.89Å c: 183.107Å
α: 90° β: 96.06° γ: 90°
R-values:
R R work R free
0.243 0.243 0.292
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Structure-function relationships among RNA-dependent RNA polymerases.
Ng et al. (2008)
14 more

7 mentions without citation

Picornaviral polymerase structure, function, and fidelity modulation.
Peersen OB. (2017)
6 more