› 1tq5

X-ray diffraction
1.76Å resolution

Crystal Structure of YhhW from Escherichia coli

Released:
DOI: 10.2210/pdb1tq5/pdb
PDB entry 1tq5 coloured by chain, front view.
PDB entry 1tq5 coloured by chain, side view.
PDB entry 1tq5 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural and biochemical analysis reveal pirins to possess quercetinase activity.
Adams M, Jia Z
J Biol Chem 280 28675-82 (2005)
PMID: 15951572

Function and Biology Details

Reaction catalysed: 
Quercetin + O(2) = 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO + H(+)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155520 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Quercetin 2,3-dioxygenase Chain: A
Molecule details ›
Chain: A
Length: 242 amino acids
Theoretical weight: 28 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P46852 (Residues: 1-231; Coverage: 100%)
Gene names: JW3402, b3439, yhhW
Sequence domains:
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand CD 6 x CD
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P3221
Unit cell:
a: 62.769Å b: 62.769Å c: 98.053Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.197 0.25
Expression system: Escherichia coli

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Citations

7 review citations

Structural studies on 2-oxoglutarate oxygenases and related double-stranded beta-helix fold proteins.
Clifton et al. (2006)
6 more

3 mentions without citation

Structure-based phylogeny as a diagnostic for functional characterization of proteins with a cupin fold.
Agarwal et al. (2009)
2 more