› 1tq9

X-ray diffraction
2Å resolution

Non-covalent swapped dimer of Bovine Seminal Ribonuclease in complex with 2'-DEOXYCYTIDINE-2'-DEOXYADENOSINE-3',5'-MONOPHOSPHATE

Released:
DOI: 10.2210/pdb1tq9/pdb
PDB entry 1tq9 coloured by chain, front view.
PDB entry 1tq9 coloured by chain, side view.
PDB entry 1tq9 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structure and stability of the non-covalent swapped dimer of bovine seminal ribonuclease: an enzyme tailored to evade ribonuclease protein inhibitor.
Sica F, Di Fiore A, Merlino A, Mazzarella L
J Biol Chem 279 36753-60 (2004)
PMID: 15192098

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132818 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Seminal ribonuclease Chains: A, B
Molecule details ›
Chains: A, B
Length: 124 amino acids
Theoretical weight: 13.75 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00669 (Residues: 27-150; Coverage: 100%)
Gene name: SRN
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:
Ligand CPA 2 x CPA
1 modified residue:
Ligand YCM 4 x YCM

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P212121
Unit cell:
a: 41.471Å b: 69.689Å c: 110.804Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.206 0.269

Quick links

Citations

4 review citations

The RNase a superfamily: generation of diversity and innate host defense.
Dyer et al. (2006)
3 more