› 1tqq

X-ray diffraction
2.75Å resolution

Structure of TolC in complex with hexamminecobalt

Released:
DOI: 10.2210/pdb1tqq/pdb
PDB entry 1tqq coloured by chain, front view.
PDB entry 1tqq coloured by chain, side view.
PDB entry 1tqq coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of the ligand-blocked periplasmic entrance of the bacterial multidrug efflux protein TolC.
Higgins MK, Eswaran J, Edwards P, Schertler GF, Hughes C, Koronakis V
J Mol Biol 342 697-702 (2004)
PMID: 15342230

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136391 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer membrane protein TolC Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 471 amino acids
Theoretical weight: 51.51 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P02930 (Residues: 23-493; Coverage: 100%)
Gene names: JW5503, b3035, colE1-i, mtcB, mukA, refI, toc, tolC, weeA
Sequence domains: Outer membrane efflux protein
Structure domains: Outer membrane efflux proteins (OEP)

Ligands and Environments

1 bound ligand:
Ligand NCO 1 x NCO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: R3
Unit cell:
a: 265.276Å b: 265.276Å c: 96.253Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.266 0.264 0.305
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Efflux-mediated antimicrobial resistance.
Poole K. (2005)
13 more

6 mentions without citation

The bacterial outer membrane β-barrel assembly machinery.
Kim et al. (2012)
5 more