› 1trb

X-ray diffraction
2Å resolution

CONVERGENT EVOLUTION OF SIMILAR FUNCTION IN TWO STRUCTURALLY DIVERGENT ENZYMES

Released:
DOI: 10.2210/pdb1trb/pdb
PDB entry 1trb coloured by chain, front view.
PDB entry 1trb coloured by chain, side view.
PDB entry 1trb coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Convergent evolution of similar function in two structurally divergent enzymes.
Kuriyan J, Krishna TS, Wong L, Guenther B, Pahler A, Williams CH, Model P
Nature 352 172-4 (1991)
PMID: 2067578

Function and Biology Details

Reaction catalysed: 
Thioredoxin + NADP(+) = thioredoxin disulfide + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141909 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin reductase Chain: A
Molecule details ›
Chain: A
Length: 320 amino acids
Theoretical weight: 34.51 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P0A9P4 (Residues: 2-321; Coverage: 100%)
Gene names: JW0871, b0888, trxB
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P6322
Unit cell:
a: 123.1Å b: 123.1Å c: 81.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.177 not available
Expression system: Not provided

Quick links

Citations

18 review citations

Thioredoxin reductase two modes of catalysis have evolved.
Williams et al. (2000)
17 more

7 mentions without citation

Predicting protein function and binding profile via matching of local evolutionary and geometric surface patterns.
Tseng et al. (2009)
6 more