› 1tro

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF TRP REPRESSOR OPERATOR COMPLEX AT ATOMIC RESOLUTION

Released:
DOI: 10.2210/pdb1tro/pdb
PDB entry 1tro coloured by chain, front view.
PDB entry 1tro coloured by chain, side view.
PDB entry 1tro coloured by chain, top view.
Source organism: Escherichia coli str. K-12 substr. W3110
Primary publication:
Crystal structure of trp repressor/operator complex at atomic resolution.
Otwinowski Z, Schevitz RW, Zhang RG, Lawson CL, Joachimiak A, Marmorstein RQ, Luisi BF, Sigler PB
Nature 335 321-9 (1988)
PMID: 3419502

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114654 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Trp operon repressor Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 108 amino acids
Theoretical weight: 12.37 KDa
Source organism: Escherichia coli str. K-12 substr. W3110
UniProt:
  • Canonical: P0A881 (Residues: 1-108; Coverage: 100%)
Gene names: JW4356, b4393, rtrY, trpR
Sequence domains: Trp repressor protein
Structure domains: TrpR-like
DNA (5'-D(*TP*GP*TP*AP*CP*TP*AP*GP*TP*TP*AP*AP*CP*TP*AP*GP*T P*AP*C)-3') Chains: I, J, K, L
Molecule details ›
Chains: I, J, K, L
Length: 19 nucleotides
Theoretical weight: 5.82 KDa
Source organism: Escherichia coli str. K-12 substr. W3110
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand TRP 4 x TRP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 43.65Å b: 72.43Å c: 107.39Å
α: 90° β: 94.96° γ: 90°
R-values:
R R work R free
0.167 not available not available
Expression system: Not provided

Quick links

Citations

68 review citations

Origins of specificity in protein-DNA recognition.
Rohs et al. (2010)
67 more

32 mentions without citation

Making the bend: DNA tertiary structure and protein-DNA interactions.
Harteis et al. (2014)
31 more