› 1ttv

Solution NMR

NMR Structure of a Complex Between MDM2 and a Small Molecule Inhibitor

Released:
DOI: 10.2210/pdb1ttv/pdb
PDB entry 1ttv coloured by chain, ensemble of 18 models, front view.
PDB entry 1ttv coloured by chain, ensemble of 18 models, side view.
PDB entry 1ttv coloured by chain, ensemble of 18 models, top view.
Source organism: Xenopus laevis
Primary publication:
NMR structure of a complex between MDM2 and a small molecule inhibitor.
Fry DC, Emerson SD, Palme S, Vu BT, Liu CM, Podlaski F
J Biomol NMR 30 163-73 (2004)
PMID: 15557803

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157449 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase Mdm2 Chain: A
Molecule details ›
Chain: A
Length: 107 amino acids
Theoretical weight: 12.21 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P56273 (Residues: 13-119; Coverage: 23%)
Gene name: mdm2
Sequence domains: SWIB/MDM2 domain
Structure domains: SWIB/MDM2 domain

Ligands and Environments

1 bound ligand:
Ligand IMY 1 x IMY
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli BL21

Quick links

Citations

13 review citations

Reaching for high-hanging fruit in drug discovery at protein-protein interfaces.
Wells et al. (2007)
12 more

3 mentions without citation

Atomic analysis of protein-protein interfaces with known inhibitors: the 2P2I database.
Bourgeas et al. (2010)
2 more