› 1tuu

X-ray diffraction
2.5Å resolution

Acetate Kinase crystallized with ATPgS

Released:
DOI: 10.2210/pdb1tuu/pdb
PDB entry 1tuu coloured by chain, front view.
PDB entry 1tuu coloured by chain, side view.
PDB entry 1tuu coloured by chain, top view.
Source organism: Methanosarcina thermophila
Primary publication:
Structural and kinetic analyses of arginine residues in the active site of the acetate kinase from Methanosarcina thermophila.
Gorrell A, Lawrence SH, Ferry JG
J Biol Chem 280 10731-42 (2005)
PMID: 15647264

Function and Biology Details

Reaction catalysed: 
ATP + acetate = ADP + acetyl phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153660 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 399 amino acids
Theoretical weight: 43.41 KDa
Source organism: Methanosarcina thermophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38502 (Residues: 1-399; Coverage: 98%)
Gene names: ack, ackA
Sequence domains: Acetokinase family
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

5 bound ligands:
Ligand SO4 1 x SO4
Ligand ADP 1 x ADP
Ligand NH4 1 x NH4
Ligand AMP 1 x AMP
Ligand PIS 1 x PIS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: C2
Unit cell:
a: 179.083Å b: 66.111Å c: 81.666Å
α: 90° β: 103.31° γ: 90°
R-values:
R R work R free
0.213 0.213 0.276
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Acetogenesis and the Wood-Ljungdahl pathway of CO(2) fixation.
Ragsdale et al. (2008)
5 more

2 mentions without citation

The EutQ and EutP proteins are novel acetate kinases involved in ethanolamine catabolism: physiological implications for the function of the ethanolamine metabolosome in Salmonella enterica.
Moore et al. (2016)
1 more