› 1tve

X-ray diffraction
3Å resolution

Homoserine Dehydrogenase in complex with 4-(4-hydroxy-3-isopropylphenylthio)-2-isopropylphenol

Released:
DOI: 10.2210/pdb1tve/pdb
PDB entry 1tve coloured by chain, front view.
PDB entry 1tve coloured by chain, side view.
PDB entry 1tve coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
New phenolic inhibitors of yeast homoserine dehydrogenase.
Ejim L, Mirza IA, Capone C, Nazi I, Jenkins S, Chee GL, Berghuis AM, Wright GD
Bioorg Med Chem 12 3825-30 (2004)
PMID: 15210149

Function and Biology Details

Reaction catalysed: 
L-homoserine + NAD(P)(+) = L-aspartate 4-semialdehyde + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151909 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Homoserine dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 358 amino acids
Theoretical weight: 38.41 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P31116 (Residues: 2-359; Coverage: 100%)
Gene names: HOM6, J2132, YJR139C
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 178 2 x 178
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P21
Unit cell:
a: 72.456Å b: 54.379Å c: 92.497Å
α: 90° β: 98.97° γ: 90°
R-values:
R R work R free
0.289 0.289 0.363
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Inhibitors of amino acids biosynthesis as antifungal agents.
Jastrzębowska et al. (2015)
1 more

5 mentions without citation

A robust bulk-solvent correction and anisotropic scaling procedure.
Afonine et al. (2005)
4 more