1tvm

Solution NMR

NMR structure of enzyme GatB of the galactitol-specific phosphoenolpyruvate-dependent phosphotransferase system

Released:

Function and Biology Details

Reaction catalysed:
[Protein]-N(pi)-phospho-L-histidine + galactitol(Side 1) = [protein]-L-histidine + galactitol 1-phosphate(Side 2)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-141252 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PTS system galactitol-specific EIIB component Chain: A
Molecule details ›
Chain: A
Length: 113 amino acids
Theoretical weight: 12.48 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A435 (Residues: 2-94; Coverage: 99%)
Gene names: c2618, gatB
Sequence domains: PTS system, Lactose/Cellobiose specific IIB subunit
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)