› 1txo

X-ray diffraction
1.95Å resolution

Crystal structure of the Mycobacterium tuberculosis serine/threonine phosphatase PstP/Ppp at 1.95 A.

Released:
DOI: 10.2210/pdb1txo/pdb
PDB entry 1txo coloured by chain, front view.
PDB entry 1txo coloured by chain, side view.
PDB entry 1txo coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
An alternate conformation and a third metal in PstP/Ppp, the M. tuberculosis PP2C-Family Ser/Thr protein phosphatase.
Pullen KE, Ng HL, Sung PY, Good MC, Smith SM, Alber T
Structure 12 1947-54 (2004)
PMID: 15530359

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161595 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine protein phosphatase PstP Chains: A, B
Molecule details ›
Chains: A, B
Length: 237 amino acids
Theoretical weight: 25.48 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHW5 (Residues: 4-240; Coverage: 46%)
Gene names: Rv0018c, mstp, ppp, pstP
Sequence domains: Protein phosphatase 2C
Structure domains: PPM-type phosphatase domain

Ligands and Environments

1 bound ligand:
Ligand MN 6 x MN
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 67.638Å b: 58.757Å c: 73.815Å
α: 90° β: 102.15° γ: 90°
R-values:
R R work R free
0.225 0.199 0.23
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Serine/threonine phosphatases: mechanism through structure.
Shi Y. (2009)
7 more

12 mentions without citation

Hanks-Type Serine/Threonine Protein Kinases and Phosphatases in Bacteria: Roles in Signaling and Adaptation to Various Environments.
Janczarek et al. (2018)
11 more