› 1txx

X-ray diffraction
2.2Å resolution

ACTIVE-SITE VARIANT OF E.COLI THIOREDOXIN

Released:
DOI: 10.2210/pdb1txx/pdb
PDB entry 1txx coloured by chain, front view.
PDB entry 1txx coloured by chain, side view.
PDB entry 1txx coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The CXXC motif: crystal structure of an active-site variant of Escherichia coli thioredoxin.
Schultz LW, Chivers PT, Raines RT
Acta Crystallogr D Biol Crystallogr 55 1533-8 (1999)
PMID: 10489448

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141959 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin 1 Chain: A
Molecule details ›
Chain: A
Length: 108 amino acids
Theoretical weight: 11.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AA25 (Residues: 2-109; Coverage: 99%)
Gene names: JW5856, b3781, fipA, trxA, tsnC
Sequence domains: Thioredoxin
Structure domains: Glutaredoxin

Ligands and Environments

1 bound ligand:
Ligand CU 1 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P43212
Unit cell:
a: 42.53Å b: 42.53Å c: 105.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.171 not available
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Structure, function, and mechanism of thioredoxin proteins.
Collet et al. (2010)
2 more

3 mentions without citation

Thioredoxin as a fusion tag for carrier-driven crystallization.
Corsini et al. (2008)
2 more