› 1tz6

X-ray diffraction
2.7Å resolution

Crystal structure of aminoimidazole riboside kinase from Salmonella enterica complexed with aminoimidazole riboside and ATP analog

Released:
DOI: 10.2210/pdb1tz6/pdb
PDB entry 1tz6 coloured by chain, front view.
PDB entry 1tz6 coloured by chain, side view.
PDB entry 1tz6 coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Primary publication:
Crystal structure of an aminoimidazole riboside kinase from Salmonella enterica: implications for the evolution of the ribokinase superfamily.
Zhang Y, Dougherty M, Downs DM, Ealick SE
Structure 12 1809-21 (2004)
PMID: 15458630

Function and Biology Details

Reaction catalysed: 
ATP + 5-amino-1-(beta-D-ribosyl)imidazole = ADP + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187240 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoimidazole riboside kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 339 amino acids
Theoretical weight: 36.44 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8ZKR2 (Residues: 1-319; Coverage: 100%)
Gene name: STM4066
Sequence domains: pfkB family carbohydrate kinase
Structure domains: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase

Ligands and Environments

4 bound ligands:
Ligand MG 2 x MG
Ligand K 4 x K
Ligand ACP 2 x ACP
Ligand AIS 1 x AIS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 137.007Å b: 54.101Å c: 89.895Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.217 0.285
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Role of Na+ and K+ in enzyme function.
Page et al. (2006)
3 more

1 mention without citation

A ribokinase family conserved monovalent cation binding site enhances the MgATP-induced inhibition in E. coli phosphofructokinase-2.
Baez et al. (2013)