› 1tzg

X-ray diffraction
2.2Å resolution

Crystal structure of HIV-1 neutralizing human Fab 4E10 in complex with a 13-residue peptide containing the 4E10 epitope on gp41

Released:
DOI: 10.2210/pdb1tzg/pdb
PDB entry 1tzg coloured by chain, front view.
PDB entry 1tzg coloured by chain, side view.
PDB entry 1tzg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Broadly neutralizing anti-HIV antibody 4E10 recognizes a helical conformation of a highly conserved fusion-associated motif in gp41.
Cardoso RM, Zwick MB, Stanfield RL, Kunert R, Binley JM, Katinger H, Burton DR, Wilson IA
Immunity 22 163-73 (2005)
PMID: 15723805

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero hexamer
PDBe Complex ID:
PDB-CPX-211572 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab 4E10 Chains: L, M
Molecule details ›
Chains: L, M
Length: 214 amino acids
Theoretical weight: 23.29 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
Fab 4E10 Chains: H, I
Molecule details ›
Chains: H, I
Length: 232 amino acids
Theoretical weight: 24.42 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
Envelope polyprotein GP160 Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 13 amino acids
Theoretical weight: 1.65 KDa

Ligands and Environments

1 bound ligand:
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2
Unit cell:
a: 157.3Å b: 45.1Å c: 198.5Å
α: 90° β: 113.8° γ: 90°
R-values:
R R work R free
0.217 0.217 0.26
Expression system: Cricetulus griseus

Quick links

Citations

69 review citations

Engineered antibody fragments and the rise of single domains.
Holliger et al. (2005)
68 more

13 mentions without citation

Interfacial pre-transmembrane domains in viral proteins promoting membrane fusion and fission.
Lorizate et al. (2008)
12 more