› 1tzo

X-ray diffraction
3.6Å resolution

Crystal Structure of the Anthrax Toxin Protective Antigen Heptameric Prepore

Released:
DOI: 10.2210/pdb1tzo/pdb
PDB entry 1tzo coloured by chain, front view.
PDB entry 1tzo coloured by chain, side view.
PDB entry 1tzo coloured by chain, top view.
Source organism: Bacillus anthracis str. A2012
Primary publication:
Structure of heptameric protective antigen bound to an anthrax toxin receptor: a role for receptor in pH-dependent pore formation.
Lacy DB, Wigelsworth DJ, Melnyk RA, Harrison SC, Collier RJ
Proc Natl Acad Sci U S A 101 13147-51 (2004)
PMID: 15326297

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146581 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protective antigen PA-63 Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, O
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, O
Length: 562 amino acids
Theoretical weight: 63.02 KDa
Source organism: Bacillus anthracis str. A2012
Expression system: Escherichia coli
UniProt:
  • Canonical: P13423 (Residues: 203-764; Coverage: 77%)
Gene names: BXA0164, GBAA_pXO1_0164, pXO1-110, pag, pagA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 28 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P1
Unit cell:
a: 149.608Å b: 167.028Å c: 168.2Å
α: 77.56° β: 75.74° γ: 76.01°
R-values:
R R work R free
0.315 0.314 0.317
Expression system: Escherichia coli

Quick links

Citations

23 review citations

Anthrax toxin: receptor binding, internalization, pore formation, and translocation.
Young et al. (2007)
22 more

27 mentions without citation

Ratcheting up protein translocation with anthrax toxin.
Feld et al. (2012)
26 more