1tzp

X-ray diffraction
1.4Å resolution

MEPA, inactive form without ZN in P21

Released:
Source organism: Escherichia coli
Primary publication:
Peptidoglycan amidase MepA is a LAS metallopeptidase.
J Biol Chem 279 43982-9 (2004)
PMID: 15292190

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-142911 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Penicillin-insensitive murein endopeptidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 255 amino acids
Theoretical weight: 28.33 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0C0T5 (Residues: 20-274; Coverage: 100%)
Gene names: JW2325, b2328, mepA
Sequence domains: Penicillin-insensitive murein endopeptidase
Structure domains: Muramoyl-pentapeptide Carboxypeptidase; domain 2

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P21
Unit cell:
a: 45.949Å b: 115.143Å c: 49.154Å
α: 90° β: 94.08° γ: 90°
R-values:
R R work R free
0.184 0.183 0.206
Expression system: Escherichia coli BL21(DE3)