› 1u07

X-ray diffraction
1.13Å resolution

Crystal Structure of the 92-residue C-term. part of TonB with significant structural changes compared to shorter fragments

Released:
DOI: 10.2210/pdb1u07/pdb
PDB entry 1u07 coloured by chain, front view.
PDB entry 1u07 coloured by chain, side view.
PDB entry 1u07 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of a 92-residue C-terminal fragment of TonB from Escherichia coli reveals significant conformational changes compared to structures of smaller TonB fragments.
Ködding J, Killig F, Polzer P, Howard SP, Diederichs K, Welte W
J Biol Chem 280 3022-8 (2005)
PMID: 15522863

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136390 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein TonB Chains: A, B
Molecule details ›
Chains: A, B
Length: 90 amino acids
Theoretical weight: 10.09 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02929 (Residues: 150-239; Coverage: 38%)
Gene names: JW5195, b1252, exbA, tonB
Sequence domains: Gram-negative bacterial TonB protein C-terminal
Structure domains: TonB

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 22.58Å b: 49.32Å c: 72.22Å
α: 90° β: 97.99° γ: 90°
R-values:
R R work R free
0.139 0.137 0.183
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Siderophore-based iron acquisition and pathogen control.
Miethke et al. (2007)
8 more

8 mentions without citation

Structure and Stoichiometry of the Ton Molecular Motor.
Celia et al. (2020)
7 more