› 1u0a

X-ray diffraction
1.64Å resolution

Crystal structure of the engineered beta-1,3-1,4-endoglucanase H(A16-M) in complex with beta-glucan tetrasaccharide

Released:
DOI: 10.2210/pdb1u0a/pdb
PDB entry 1u0a coloured by chain, front view.
PDB entry 1u0a coloured by chain, side view.
PDB entry 1u0a coloured by chain, top view.
Source organism: Paenibacillus macerans
Primary publication:
Structural basis for the substrate specificity of a Bacillus 1,3-1,4-beta-glucanase.
Gaiser OJ, Piotukh K, Ponnuswamy MN, Planas A, Borriss R, Heinemann U
J Mol Biol 357 1211-25 (2006)
PMID: 16483609

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-150105 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-glucanase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 214 amino acids
Theoretical weight: 23.93 KDa
Source organism: Paenibacillus macerans
Expression system: Escherichia coli
UniProt:
  • Canonical: P23904 (Residues: 25-237; Coverage: 100%)
Sequence domains: Glycosyl hydrolases family 16
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
2 bound ligands:
Ligand CA 4 x CA
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P212121
Unit cell:
a: 75.769Å b: 88.758Å c: 154.795Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.161 0.197
Expression system: Escherichia coli

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Citations

1 review citation

Cancer immunotherapies revisited: state of the art of conventional treatments and next-generation nanomedicines.
García-Fernández et al. (2021)
26 other articles

8 mentions without citation

Structural Survey of Zinc Containing Proteins and the Development of the Zinc AMBER Force Field (ZAFF).
Peters et al. (2010)
7 more