› 1u0b

X-ray diffraction
2.3Å resolution

Crystal structure of cysteinyl-tRNA synthetase binary complex with tRNACys

Released:
DOI: 10.2210/pdb1u0b/pdb
PDB entry 1u0b coloured by chain, front view.
PDB entry 1u0b coloured by chain, side view.
PDB entry 1u0b coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Shape-selective RNA recognition by cysteinyl-tRNA synthetase.
Hauenstein S, Zhang CM, Hou YM, Perona JJ
Nat Struct Mol Biol 11 1134-41 (2004)
PMID: 15489861

Function and Biology Details

Reaction catalysed: 
ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149466 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Cysteine--tRNA ligase Chain: B
Molecule details ›
Chain: B
Length: 461 amino acids
Theoretical weight: 52.27 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P21888 (Residues: 1-461; Coverage: 100%)
Gene names: JW0515, b0526, cysS
Sequence domains:
Structure domains: HUPs
cysteinyl tRNA Chain: A
Molecule details ›
Chain: A
Length: 74 nucleotides
Theoretical weight: 23.78 KDa
Source organism: Escherichia coli
Expression system: Not provided
Sequence domains: tRNA

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P41212
Unit cell:
a: 129.49Å b: 129.49Å c: 91.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.29 0.233 0.277
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

Structure of transfer RNAs: similarity and variability.
Giegé et al. (2012)
5 more

35 mentions without citation

Protein adaptations in archaeal extremophiles.
Reed et al. (2013)
34 more