› 1u0m

X-ray diffraction
2.22Å resolution

Crystal Structure of 1,3,6,8-Tetrahydroxynaphthalene Synthase (THNS) from Streptomyces coelicolor A3(2): a Bacterial Type III Polyketide Synthase (PKS) Provides Insights into Enzymatic Control of Reactive Polyketide Intermediates

Released:
DOI: 10.2210/pdb1u0m/pdb
PDB entry 1u0m coloured by chain, front view.
PDB entry 1u0m coloured by chain, side view.
PDB entry 1u0m coloured by chain, top view.
Source organism: Streptomyces coelicolor A3(2)
Primary publication:
Crystal structure of a bacterial type III polyketide synthase and enzymatic control of reactive polyketide intermediates.
Austin MB, Izumikawa M, Bowman ME, Udwary DW, Ferrer JL, Moore BS, Noel JP
J Biol Chem 279 45162-74 (2004)
PMID: 15265863

Function and Biology Details

Reaction catalysed: 
5 malonyl-CoA = 1,3,6,8-tetrahydroxynaphthalene + 5 CoA + 5 CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190286 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1,3,6,8-tetrahydroxynaphthalene synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 382 amino acids
Theoretical weight: 41.67 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9FCA7 (Residues: 1-374; Coverage: 100%)
Gene name: SCO1206
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

2 bound ligands:
Ligand 15P 2 x 15P
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P21
Unit cell:
a: 76.679Å b: 69.685Å c: 81.135Å
α: 90° β: 95.42° γ: 90°
R-values:
R R work R free
0.255 0.253 0.292
Expression system: Escherichia coli BL21

Quick links

Citations

24 review citations

The biosynthetic logic of polyketide diversity.
Hertweck C. (2009)
23 more

6 mentions without citation

Structural classification and properties of ketoacyl synthases.
Chen et al. (2011)
5 more