› 1u0t

X-ray diffraction
2.3Å resolution

Crystal structure of Mycobacterium tuberculosis NAD kinase

Released:
DOI: 10.2210/pdb1u0t/pdb
PDB entry 1u0t coloured by chain, front view.
PDB entry 1u0t coloured by chain, side view.
PDB entry 1u0t coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
A novel fold revealed by Mycobacterium tuberculosis NAD kinase, a key allosteric enzyme in NADP biosynthesis.
Garavaglia S, Raffaelli N, Finaurini L, Magni G, Rizzi M
J Biol Chem 279 40980-6 (2004)
PMID: 15269221

Function and Biology Details

Reaction catalysed: 
ATP + NAD(+) = ADP + NADP(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161593 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 307 amino acids
Theoretical weight: 32.94 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHV7 (Residues: 1-307; Coverage: 100%)
Gene names: MTCI125.17, Rv1695, ppnK
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21212
Unit cell:
a: 64.757Å b: 145.655Å c: 68.713Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.226 0.289
Expression system: Escherichia coli

Quick links

Citations

9 review citations

The power to reduce: pyridine nucleotides--small molecules with a multitude of functions.
Pollak et al. (2007)
8 more

1 mention without citation

Structural determinants of discrimination of NAD+ from NADH in yeast mitochondrial NADH kinase Pos5.
Ando et al. (2011)