› 1u10

X-ray diffraction
2.4Å resolution

MEPA, active form with ZN in P1

Released:
DOI: 10.2210/pdb1u10/pdb
PDB entry 1u10 coloured by chain, front view.
PDB entry 1u10 coloured by chain, side view.
PDB entry 1u10 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Peptidoglycan amidase MepA is a LAS metallopeptidase.
Marcyjaniak M, Odintsov SG, Sabala I, Bochtler M
J Biol Chem 279 43982-9 (2004)
PMID: 15292190

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142911 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Penicillin-insensitive murein endopeptidase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 255 amino acids
Theoretical weight: 28.61 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0C0T5 (Residues: 20-274; Coverage: 100%)
Gene names: JW2325, b2328, mepA
Sequence domains: Penicillin-insensitive murein endopeptidase
Structure domains: Muramoyl-pentapeptide Carboxypeptidase; domain 2

Ligands and Environments

2 bound ligands:
Ligand ZN 9 x ZN
Ligand SO4 6 x SO4
1 modified residue:
Ligand MSE 36 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P1
Unit cell:
a: 35.613Å b: 77.988Å c: 127.663Å
α: 93.15° β: 95.93° γ: 90.75°
R-values:
R R work R free
0.236 0.234 0.266
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Bacterial peptidoglycan (murein) hydrolases.
Vollmer et al. (2008)
6 more

2 mentions without citation

Cell Wall Hydrolases in Bacteria: Insight on the Diversity of Cell Wall Amidases, Glycosidases and Peptidases Toward Peptidoglycan.
Vermassen et al. (2019)
1 more