› 1u1j

X-ray diffraction
2.4Å resolution

A. thaliana cobalamine independent methionine synthase

Released:
DOI: 10.2210/pdb1u1j/pdb
PDB entry 1u1j coloured by chain, front view.
PDB entry 1u1j coloured by chain, side view.
PDB entry 1u1j coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Crystal structures of cobalamin-independent methionine synthase complexed with zinc, homocysteine, and methyltetrahydrofolate.
Ferrer JL, Ravanel S, Robert M, Dumas R
J Biol Chem 279 44235-8 (2004)
PMID: 15326182

Function and Biology Details

Reaction catalysed: 
5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129292 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1 Chain: A
Molecule details ›
Chain: A
Length: 765 amino acids
Theoretical weight: 85.37 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O50008 (Residues: 1-765; Coverage: 100%)
Gene names: At5g17920, CIMS, MPI7.9, MS1
Sequence domains:
Structure domains: TIM Barrel

Ligands and Environments

4 bound ligands:
Ligand ZN 2 x ZN
Ligand SO4 4 x SO4
Ligand MET 1 x MET
Ligand C2F 1 x C2F
1 modified residue:
Ligand MSE 19 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P65
Unit cell:
a: 123.465Å b: 123.465Å c: 132.721Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.215 0.275
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Methionine salvage and S-adenosylmethionine: essential links between sulfur, ethylene and polyamine biosynthesis.
Sauter et al. (2013)
4 more

4 mentions without citation

In vivo protein tyrosine nitration in Arabidopsis thaliana.
Lozano-Juste et al. (2011)
3 more